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primary human coronary artery endothelial cells hcaecs  (PromoCell)


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    PromoCell primary human coronary artery endothelial cells hcaecs
    Primary Human Coronary Artery Endothelial Cells Hcaecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/coronary+artery+endothelial+cells+hcaecs/Human+Coronary+Artery+Endothelial+Cells/pm42259138-95-0-11
    Average 96 stars, based on 228 article reviews
    primary human coronary artery endothelial cells hcaecs - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Initial contact guidance during cell spreading is contractility-independent.
    Article Snippet: .. 105 106 2.2 Cell culture 107 Human coronary artery endothelial cells (HCAECs) (Promocell, Germany), in this 108 work abbreviated as ECs, were cultured in Endothelial Cell Growth Medium 109 supplemented with Supplement Mix (Promocell, Germany) and 1% 110 penicillin/streptomycin (Life Technologies, Germany). ..

    Article Title: Carboxyl-Terminal Cleavage of Apolipoprotein A-I by Human Mast Cell Chymase Impairs Its Anti-Inflammatory Properties
    Article Snippet: .. Culture of human coronary artery endothelial cells HCAECs (PromoCell) were cultured in Endothelial Cell Growth Medium MV (Basal Medium; Catalog Number C-22220, PromoCell) supplemented with 5% fetal calf serum, 0.4% endothelial cell growth supplement, 10 ng/ml epidermal growth factor, 90 μg/ml heparin, 1 μg/ml hydrocortisone (Supplement pack, Catalog Number C-39220, PromoCell), and 100 U/mL penicillin streptomycin solution, and 50 ng/ml amphotericin B to yield Complete Medium in T-75 flask according to according to the manufacturer's instructions. ..

    Article Title: Carboxyl-Terminal Cleavage of Apolipoprotein A-I by Human Mast Cell Chymase Impairs Its Anti-Inflammatory Properties
    Article Snippet: .. Culture of human coronary artery endothelial cells HCAECs (PromoCell) were cultured in Endothelial Cell Growth Medium MV (Basal Medium; Catalog Number C-22220, PromoCell) supplemented with 5% fetal calf serum, 0.4% endothelial cell growth supplement, 10 ng/ml epidermal growth factor, 90 μg/ml heparin, 1 μg/ml hydrocortisone (Supplement pack, Catalog Number C-39220, PromoCell), and 100 U/mL penicillin streptomycin solution, and 50 ng/ml amphotericin B to yield Complete Medium in T-75 flask according to according to the manufacturer's instructions. ..

    Article Title: Divergent Regulation of Actin Dynamics and Megakaryoblastic Leukemia-1 and -2 (Mkl1/2) by cAMP in Endothelial and Smooth Muscle Cells
    Article Snippet: Smooth muscle and endothelial cell culture: Mixed donor HUVECs were purchased from Promocell and cultured in endothelial cell growth medium (supplemented with 2% FCS, 0.4 % bovine hypothalamic extract, 0.1 ng/ml EGF, 1 ng/ml bFGF, 90 μg/ml heparin and 1 μg/ml hydrocortisone). .. Three different batches of human coronary artery endothelial cells (HCAECs) were purchased from Promocell and cultured in endothelial growth medium MV2 supplemented with 5% FCS, 5 ng/ml EGF, 10 ng/ml bFGF, 20 ng/ml IGF, 0.5 ng/ml VEGF165 and 0.2 ug/ml hydrocortisone. ..

    Article Title: Chymase released from hypoxia-activated cardiac mast cells cleaves human apoA-I at Tyr192 and compromises its cardioprotective activity
    Article Snippet: .. Cultures of human coronary artery endothelial cells (HCAECs) and wound healing assay HCAECs (PromoCell) were cultured in T-75 flasks in Endothelial Cell Basal Medium MV (Basal Medium; Catalog Number C-22220, PromoCell) supplemented with 5% fetal calf serum (FCS), 0.4% endothelial cell growth supplement, 10 ng/mL epidermal growth factor, 90 μg/mL heparin, 1 μg/mL hydrocortisone (all as components of Supplement pack, Catalog Number C-39220, PromoCell), 100 U/mL penicillin/streptomycin solution, and 50 ng/mL amphotericin B to yield Complete Medium according to the instructions of the manufacturer. ..

    Wound Healing Assay:

    Article Title: Chymase released from hypoxia-activated cardiac mast cells cleaves human apoA-I at Tyr192 and compromises its cardioprotective activity
    Article Snippet: .. Cultures of human coronary artery endothelial cells (HCAECs) and wound healing assay HCAECs (PromoCell) were cultured in T-75 flasks in Endothelial Cell Basal Medium MV (Basal Medium; Catalog Number C-22220, PromoCell) supplemented with 5% fetal calf serum (FCS), 0.4% endothelial cell growth supplement, 10 ng/mL epidermal growth factor, 90 μg/mL heparin, 1 μg/mL hydrocortisone (all as components of Supplement pack, Catalog Number C-39220, PromoCell), 100 U/mL penicillin/streptomycin solution, and 50 ng/mL amphotericin B to yield Complete Medium according to the instructions of the manufacturer. ..

    Isolation:

    Article Title: Cell-specific expression of uptake transporters--a potential approach for cardiovascular drug delivery devices.
    Article Snippet: Enhanced proliferation of human coronary artery smooth muscle cells (HCASMCs) and thereby formation of neointima is one of the factors contributing to failure of coronary stents.. Even if the use of drug eluting stents (DES) and thereby the local delivery of cytotoxic compounds has significantly improved the clinical outcome, unselective cytotoxic effects are assumed to hamper clinical success.. Novel pharmacological approaches are required to enhance cellular selectivity of locally delivered drugs.



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    Image Search Results


    MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected HCAECs was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected HCAECs was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Expressing, Infection, Quantitative RT-PCR, Western Blot

    UK5099 alleviates MRMP-induced mitochondrial damage (A) mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey’s post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: UK5099 alleviates MRMP-induced mitochondrial damage (A) mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey’s post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Infection, Quantitative RT-PCR, Expressing, Western Blot

    UK5099 alleviates MRMP-induced pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay. (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: UK5099 alleviates MRMP-induced pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay. (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Expressing, Infection, Western Blot

    MG132-mediated PINK1 deficiency induces mitochondrial damage. (A) Mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E,F) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MG132-mediated PINK1 deficiency induces mitochondrial damage. (A) Mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E,F) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Infection, Quantitative RT-PCR, Expressing, Western Blot

    MG132-mediated PINK1 deficiency induces pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay ( n = 3). (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MG132-mediated PINK1 deficiency induces pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay ( n = 3). (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Expressing, Infection, Western Blot

    MPC1 induces pyroptosis of HCAECs via inhibiting PINK1-dependent mitophagy. (A–D) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). (E,F) Cytokine release was determined using ELISA ( n = 3). (G) Cell viability was determined using CCK-8 assay ( n = 3). (H) Cytotoxicity was determined using LDH assay ( n = 3). (I,J) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (K–N) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MPC1 induces pyroptosis of HCAECs via inhibiting PINK1-dependent mitophagy. (A–D) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). (E,F) Cytokine release was determined using ELISA ( n = 3). (G) Cell viability was determined using CCK-8 assay ( n = 3). (H) Cytotoxicity was determined using LDH assay ( n = 3). (I,J) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (K–N) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Expressing, Infection, Western Blot, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay